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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X <t>0,16S</t> values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.
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Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X 0,16S values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.

Journal: Environmental Microbiology

Article Title: The Adult Aedes aegypti Bacterial Microbiota is Robust to Infection with the Obligate Microsporidian Parasite Edhazardia aedis

doi: 10.1111/1462-2920.70437

Figure Lengend Snippet: Infection with E. aedis does not alter bacterial load in the abdomens of A. aegypti . The y ‐axis displays log10‐transformed ∆ X 0,16S values, which were obtained from analysis of quantitative PCR data, and represent the starting amount of 16S bacterial DNA in each sample normalized to the amount of RpS7 reference mosquito DNA. ∆ X 0,16S values serve as a proxy for measurement of bacterial load. The p value indicates the result of a mixed linear model testing the effect of E. aedis infection on log10‐transformed ∆ X 0,16S values.

Article Snippet: Amplicons were submitted to NC State Genomic Sciences Laboratory for subsequent library preparation following the Illumina 16S Metagenomic Sequencing Library Preparation guide ( 16S Metagenomic Sequencing Library Preparation: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System ).

Techniques: Infection, Transformation Assay, Analysis, Real-time Polymerase Chain Reaction